human melanoma cell lines skmel28  (ATCC)

Journal: Genes & Development

Article Title: Fatty acid uptake activates an AXL–CAV1–β-catenin axis to drive melanoma progression

doi: 10.1101/gad.351985.124

Figure Lengend Snippet: Reagents used in this study

Article Snippet: SKmel28 (male) human melanoma cell line , ATCC , , CVCL_0526.

Techniques: Software, Transduction, Virus, Subcloning, Bacteria, Recombinant, Bradford Assay, Protease Inhibitor, RNA Extraction, Reporter Assay, Bicinchoninic Acid Protein Assay, Quantitative RT-PCR, Negative Control

Journal: Cell communication and signaling : CCS

Article Title: Revealing the role of RAB27 in HER receptor family expression and signaling in melanoma cells.

doi: 10.1186/s12964-025-02064-8

Figure Lengend Snippet: Fig. 1 Expression of cancer-related proteins in wild-type (WT) and RAB27A knockout (KO) SkMel28, DMBC12 and A375 cells. Signal intensity was quanti fied by densitometric analysis and data are presented as mean values of two technical replicates. Proteins whose signal exceeded the OD value of 3000 and the difference between WT and KO was at least 20% were grouped according to their function

Article Snippet: The human melanoma SkMel28 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Knock-Out

Journal: Cell communication and signaling : CCS

Article Title: Revealing the role of RAB27 in HER receptor family expression and signaling in melanoma cells.

doi: 10.1186/s12964-025-02064-8

Figure Lengend Snippet: Fig. 3 Expression of HER receptors in wild-type (WT) and RAB27A knockout (KO) SkMel28, DMBC12 and A375 cells and RAB27A/B knockout (dKO) A375 cells measured using the Proteome Profiler array. Signal intensity was quantified by densitometric analysis and data are presented as mean OD values of two technical replicates

Article Snippet: The human melanoma SkMel28 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Knock-Out

Journal: Cell communication and signaling : CCS

Article Title: Revealing the role of RAB27 in HER receptor family expression and signaling in melanoma cells.

doi: 10.1186/s12964-025-02064-8

Figure Lengend Snippet: Fig. 4 RAB27A knockout altered the levels of EGFR, HER2 and HER3 in wild-type (WT) and RAB27A knockout (KO) SkMel28, DMBC12 and A375 cells. (A) mRNA expression analyzed by RT-PCR. Data presented as mean ± SD, n ≥ 3, p < 0.05 (B) Receptor occupancy analyzed by flow cytometry. Data presented as mean ± SD, n ≥ 4, p < 0.05 (C) Representative flow cytometry (D) Protein expression analysed by Western blot. The acquired bands were quantified by densitometric analysis. Data presented as mean ± SD, n ≥ 5, p < 0.05 (E) Representative Western blot

Article Snippet: The human melanoma SkMel28 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Knock-Out, Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, Western Blot

Journal: International journal of molecular sciences

Article Title: Impact of Rab27 on Melanoma Cell Invasion and sEV Secretion.

doi: 10.3390/ijms252212433

Figure Lengend Snippet: Figure 1. Melanoma cell lines show different levels of Rab27A mRNA and protein. The basal levels of RAB27A mRNA and Rab27A protein in SkMel28, DMBC12, and A375 melanoma cell lines were measured using qRT-PCR (A) and Western blot (B), respectively. (A) Data are presented as the mean ± SD of 3 independent experiments, * SkMel28 vs. DMBC12 or A375, p < 0.05 (one-way ANOVA with post hoc Tukey). (B) The representative Western blot of 3 independent experiments is shown. (C) The acquired bands were quantified by densitometric analysis and data are presented as the mean values ± SD of 5 independent experiments. * SkMel28 vs. DMBC12 or A375, p < 0.05 (one-way ANOVA with post hoc Tukey).

Article Snippet: The SkMel28 human melanoma cell line was purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Western Blot

Journal: International journal of molecular sciences

Article Title: Impact of Rab27 on Melanoma Cell Invasion and sEV Secretion.

doi: 10.3390/ijms252212433

Figure Lengend Snippet: Figure 2. Confirmation of RAB27A knockout in SkMel28, DMBC12 and A375 melanoma cell lines. The RAB27A mRNA and Rab27A protein levels were detected using qRT-PCR and Western blot, respectively. (A) Data are presented as the mean ± SD of 3 independent experiments, * WT vs. KO, p < 0.05 (unpaired t-test). (B) The representative Western blot of 3 independent experiments is shown.

Article Snippet: The SkMel28 human melanoma cell line was purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Knock-Out, Quantitative RT-PCR, Western Blot

Journal: International journal of molecular sciences

Article Title: Impact of Rab27 on Melanoma Cell Invasion and sEV Secretion.

doi: 10.3390/ijms252212433

Figure Lengend Snippet: Figure 4. Characterization of sEVs secreted by WT and RAB27A knockout SkMel28, DMBC12, and A375 cells. (A–C) The size distribution and concentration of the secreted sEVs were analyzed by NTA. (A) Images of representative particle measurements are shown. The size (B) and concentration (C) of the particles are presented as the mean ± SD of at least 4 independent experiments, ** WT and KO A375 sEVs vs. WT and KO SkMel28 and DMBC12 sEVs, p < 0.05 # WT and KO SkMel28 sEVs vs. WT and KO DMBC12 and A375 sEVs, p < 0.05 (one-way ANOVA with post hoc Tukey). (D) The total protein concentration of the sEVs resuspended in equal volumes of PBS was measured by BCA assay. Data are presented as the mean ± SD of at least 4 independent experiments. * WT vs. KO, p < 0.05 (unpaired t-test) (E) The total protein content of the sEVs was also visualized by silver staining of SDS-PAGE. The representative images of 3 independent experiments are presented.

Article Snippet: The SkMel28 human melanoma cell line was purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Knock-Out, Concentration Assay, Protein Concentration, BIA-KA, Silver Staining, SDS Page